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HiT-Me (for High Throughput Metabolic phenotyping) is a unique facility enabling the quantification of metabolites and enzyme activities in very large numbers of samples. Various metabolic traits (key metabolites and most of the central metabolism and redox enzymes) can be measured in most plant matrices with high throughput thanks to laboratory automation. 

HiT-Me allows high throughput targeted assays for key metabolites and enzyme activities from carbon and nitrogen central metabolism, and redox metabolism. In collaboration with the MetaboHUB infrastructure, the installation developed a new non-targeted metabolomics (using LC-Orbitrap) high throughput analysis pipeline, significantly increasing the number of measured analytes, including secondary metabolites. HiT-Me develops and optimises assays for most tissue/organ/species. Examples of application include quantitative genetics, metabolic physiology, diagnostics, metabolic biomarkers, modelling of metabolism (parameterisation).

Equipment involves storage at -80°C, three cryogenic grinding units, one robot dedicated to cryogenic weighing of aliquots, two 96-channel liquid handling robots, one integrated robotic unit (96-channel liquid handling, centrifuge, heating, microplate reading, etc.), 11 filter-based microplate readers, one microplate spectrophotometer-fluorimeter-luminometer and one LIMS. The installation, originally based on microplate technology, is now turning to microfluidics to increase the possibilities of multiplexing.

Contact: Yves Gibon

Integrated robots for high throughput pipetting